Search results for " seed train"
Article
Single-Use Technologies Prove Effective for Viral Vector Process Development
Flatware is often used in the seed train for larger-scale processes as well as for small-scale production, according to Tan. T-flasks, for instance, are generally used for subculture, while cell facto…
Article
Commercial Production of Gene Therapies Using Suspension or Adherent Cell Lines
For suspension cells in STRs, the seed train is straightforward: an inoculum is prepared in a shake flask and gradually increased in volume to bioreactors, usually up to 10-fold at a time, until it re…
Article
An Integrated Approach to Ensure the Viral Safety of Biotherapeutics
Testing product and process intermediates alone is helpful, but does not provide a complete solution to viral safety. This article proposes integrated solutions for systemic and proactive viral risk m…
Article
Perfusion N-1 – A Way to Improve the Fed-Batch Production Process
Click here to read more >>
In this article, we show how the conventional N-1 seed train step was replaced by a high-density perfusion and how the production bioreactor can be seeded at a 10-fol…
Article
Single-use Bioreactors Have Reached the Big Time
In addition, Norris notes that while early adopters limited use of disposable bioreactors to process development or seed train processes feeding large stainless-steel bioreactors due to the 2000 L pea…
Article
Intensifying your fed-batch process using cryobags
We will show that high-density cryobags can be used to seed a bioreactor at a 500-fold higher seed density, reducing the total process time by up to 9 days.
READ FULL…
Article
Improving Upstream Predictability
Currently, he says, 50–60% of biopharmaceutical developers and manufacturers have either implemented or are considering use of perfusion for seed train or production bioreactor steps. Used with high c…
Article
Mapping a Route for Cell and Gene Therapy Process Development
While cell and gene therapies differ in many ways, some of the best practices for process development and validation are similar.
By Cynthia A. Challener
…
Article
Automating the Biomanufacturing Process
As automation in biomanufacturing becomes more important, so does the need to integrate process data.
By Feliza Mirasol
unlimit3d/Stock.Adobe.com
Biomanufacturing proc…
Article
Designing a Biomanufacturing Facility Incorporating Single-Use Technologies
Asking the right questions is crucial to establishing a facility design.
By Peter Genest, John Joseph
The benefits of adopting single-use technologies in the production of biopharmaceuticals…